Spectra for different fluorophores
Compare the excitation and emission spectra of multiple probes together with this Molecular Probes tool:
This one from the University of Arizona is pretty cool too.
Protocols for microscopy
online protocols guide
Free image analysis software:
EZC1 Viewer - Free basic version of Nikon's confocal software
Image J - Free image
processing software that can do almost anything you need (make sure you
check out the Plugins)
Nyquist Calculator - Online calculator to work out the optical slice thickness and optimal step size.
General Microscopy/Confocal Information
Molecular probes: http://probes.invitrogen.com/
If it's fluorescent, they've probably got it. There are also some excellent resources, including online tutorials.
Microscopy entry at Wikipedia.
An excellent source of microscopy info.
Everything you ever wanted to know about light microscopy but were too lazy to ask
Molecular Expressions: http://micro.magnet.fsu.edu/primer/index.html
Similar to MicroscopyU
Zeiss - Microscopy from the beginning:
General microscopy info and history of microscopy
Microscopy UK: http://www.microscopy-uk.org.uk/mag/indexmag.html
A fun site with photo galleries and handy
DIY hints for adventurous microscopists
For the physics-minded:
How confocal microscopy works
Confocal Listserv: http://listserv.buffalo.edu/archives/confocal.html
A listserv for confocal users - good for getting obscure or very technical information about confocal microscopy
Journals & Magazines
Microscopy magazines: http://swehsc.pharmacy.arizona.edu/exppath/micro/freemags.html
This page has an extensive list of free microscopy magazines - both online and print - that you can subscribe to
Nature Reviews - focus on fluorescence imaging -
Some of these other labs and facilities have some great info on microscopy and their research, as well as cool images.
Jim Hasseloff's Lab:
Confocal Facility at Ludwig Institute for Cancer Research:
WM Keck Laboratory for Biological Imaging:
Murphy, Douglas B. (2001) Fundamentals of light microscopy and electronic imaging. Wiley-Liss
(Library Call #
QH211 .M87 2001)
Herman, B. (1997) Fluorescence Microscopy (2nd edition). Bios
Scientific Publishers. (Library Call #:
QH212.F55 H47 1998)
Pawley, James B. (2006) Handbook of Biological Confocal
Microscopy (3rd edition). Springer. (Library Call #:
QH224 .H36 2006 )